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KMID : 0848120130380040141
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International Journal of Oral Biology
2013 Volume.38 No. 4 p.141 ~ p.147
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Antimicrobial Activity of Berberine against Oral Bacteria Related to Endodontic Infections
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Kook Joong-Ki
Lee Dong-kyun
Kim Min-Jung
Park Soon-Nang
Lim Yun-Kyong
Min Jeong-Beom
Hwang Ho-Keel
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Abstract
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It has been established that berberine has strongantimicrobial effects. Little is known however regarding theantimicrobial activity of berberine against endodonticpathogenic bacteria or its cytotoxicity in human oral tissuecells. The antibacterial properties of berberine were testedagainst 5 strains of Enterococcus faecalis and type strains ofAggregatibacter actinomycetemcomitans, Prevotellanigrescens, Prevotella intermedia, and Tannerella forsythia,which are involved in endodontic infections. Antimicrobialactivity was evaluated through minimum inhibitoryconcentration (MIC) and minimum bactericidal concentration(MBC) measurements. The viability of normal humangingival fibroblast (NHGF) cells after exposure to berberinewas measured using a methyl thiazolyl tetrazolium (MTT)assay. The data showed that berberine has antimicrobialeffects against A. actinomycetemcomitans with an MIC andMBC of 12.5 μg/ml and 25 μg/ml, respectively. In thecytotoxicity studies, cell viability was maintained at 66.1%following exposure to 31.3 μg/ml berberine. Overall, thesefindings suggest that berberine has antimicrobial activityagainst the tested bacteria. Nevertheless, lower concentrationsin combination with other reagents will need to be testedbefore these in vitro results can be translated to clinical use.
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KEYWORD
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Antimicrobial effect, berberine, endodontic infection
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